The following data is an RT-PCR experiment comparing expression of the indicated genes in iPS cells (induced pluripotent stem cells), ES cells (embryonic stem cells) and MEFs (mouse embryonic fibroblasts - differentiated cells).
Nat1 is considered to be a loading control. What is the purpose of a loading control?
iPS-
MEF 24-1
5 9 18 S MES
Nanog
ERas
Oct3/4
Sox2
Fgf4
Cripto
Dax 1
Zfp296
Nat1
RT minus
a. To show that all cells have mRNA.
b. To show that the probes being used to detect all of the indicated genes work.
c. To show that the different strains tested have the same gene expression profiles.
d. To show that equal amounts of RNA were used in the RT-PCR reactions.
Answers
The purpose of a loading control in an RT-PCR experiment is to show that equal amounts of RNA were used in the RT-PCR reactions.
In RT-PCR experiments, a loading control is a reference gene or sequence that is used to ensure that an equal amount of RNA was added to each sample during the reverse transcription and PCR processes. By including a loading control, researchers can normalize the expression levels of their target genes to the total amount of RNA present in each sample. This helps account for any variations in RNA quantity or quality among the samples.
In this specific experiment comparing gene expression in iPS cells, ES cells, and MEFs, the gene Nat1 is being used as the loading control. By including Nat1 in the analysis, the researchers can verify that the same amount of RNA was used for all samples, allowing for accurate comparisons of gene expression levels between the different cell types.
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Related Questions
an intranet is group of answer choices an external vehicle that allows for exchange of information via a cooperatively run and globally distributed collection of computer networks an external vehicle that integrates communication with outsiders so they have more insight about workflow, process management, and infrastructure an internal vehicle that integrates communication with workflow, process management, and infrastructure a dynamic web site to which all employees can add information
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An intranet is an internal vehicle that integrates communication with workflow, process management, and infrastructure.
It is a computer network that is designed to be used within an organization or business to facilitate communication and collaboration between employees. Intranets are used to share information, manage workflows, and improve overall productivity. They are often used in large companies with multiple departments and teams, as they allow for easy communication and collaboration across different areas of the organization.Overall, an intranet is an internal vehicle that allows for the exchange of information between employees in a secure and efficient manner. It is an essential tool for organizations that want to improve their workflow, communication, and productivity.for more such question on network
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Explain a way that color is used to convey a message without using words?
Answers
Answer: using symbols and actions without words
Explanation:
Write a short-essay discussing your own stand on social media usage for students.
Answers
Social media is a prevalent part of our daily lives, and it has become an integral part of many students' lives as well. The internet is full of potential, but social media can be a double-edged sword, and its use by students is a contentious topic.
While there are pros and cons to using social media for students, my stand on social media usage for students is that it should be limited and supervised to avoid any harmful consequences.The benefits of social media for students are numerous. It can help students to connect and communicate with their peers and teachers, collaborate on group projects, and share ideas and resources.
Social media can also be a great source of information and can help students to stay up-to-date on current events and trends. However, the drawbacks of social media usage cannot be overlooked. Excessive use of social media can lead to addiction and interfere with academics, socialization, and overall well-being. Moreover, social media is also linked to cyberbullying, online harassment, and exposure to inappropriate content.
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Phase One Start by building an 8-bit ALU using Logisim. This ALU can implement 16 instructions on 8-bit operands. We would suggest the following minimum list of instructions: Arithmetic addition Increment Decrement Comparison (with 3 outputs: one for equals, one for less than and one for greater than) Logic bitwise Not Logic bitwise And Logic bitwise Or Register right logic shift Register left logic shift In addition to these nine instructions, please suggest ve more instructions that the ALU can implement for a total of 14 instructions (we are reserving 2 instructions for branching). Justify the importance of the ve instructions you added in a Word doc to submitted as part of this assignment. Label these instructions as 'Phase One.' After you've suggested and justied your ve suggested instructions, please build at least the nine above-mentioned operations as blocks in Logisim.
Answers
The five additional instructions for the 8-bit ALU are:
Bitwise XOR - For logical operations on binary data.Clear register - To reset a register's value to zero.Load immediate - For loading a constant value into a register directly.Rotate left - Shifts bits to the left and wraps them around.Rotate right - Shifts bits to the right and wraps them around.Here are five additional instructions that can be implemented in the 8-bit ALU, along with their justifications:
Bitwise XOR (exclusive OR): This instruction is important for performing logical operations on binary data. XOR allows for toggling or flipping specific bits, which is useful in various applications such as data encryption and error detection.Clear register: The clear register instruction resets the value of a register to zero. This is valuable for initializing registers before performing calculations or clearing data when needed, ensuring accurate and predictable results.Load immediate: This instruction enables loading a constant value directly into a register. It eliminates the need for multiple instructions to load immediate values, improving efficiency and reducing program sizeRotate left: The rotate left instruction shifts the bits of a register to the left, moving the leftmost bit to the rightmost position and wrapping the shifted bits around. This operation is beneficial for circular shifting and bitwise rotation in various algorithms and data processing tasks.Rotate right: Similar to the rotate left instruction, rotate right shifts the bits of a register to the right, wrapping the shifted bits from the rightmost position to the leftmost position. This operation is valuable for symmetric bit manipulation and circular shifting.Implementing these additional instructions enhances the ALU's functionality by providing more operations for data manipulation, initialization, and bitwise rotation, expanding its capabilities in various computational tasks.
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Anyone know? I don’t know how to do this. Btw this is robotics and this is RPM
Answers
Answer:
it is the 2nd one
Explanation:
the core component of the linux operating system is the linux kernel. if you were a linux systems administrator for a company, when would you need to upgrade your linux kernel?
Answers
The Linux® kernel serves as the main interface between a computer's hardware and its processes and is a key part of the Linux operating system (OS). In order to manage resources as effectively as possible, it communicates between the two.
Which of the following approaches is typically used to get Linux support?Numerous platforms and programming languages are supported by Linux, which is widely used. HOWTO guides, a local Linux User Group (LOG), and online newsgroups are all good resources. Which two individuals are recognized as the architects of the UNIX operating system?
What does an operating system's core go by?The kernel is the fundamental building block of an operating system for a computer (OS). All other components of the OS rely on the core to provide them with essential services.
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A crucial component of the Linux operating system is the Linux® kernel, which acts as the primary communication between a desktop computer and its activities (OS).
Which of the following strategies is most frequently employed to obtain Linux support?Linux, which is extensively used, accommodates a broad range of systems and programming languages. Online communities, local Linux User Groups (LOGs), and HOWTO publications are also useful sources.
What is the name of the core of an operating system?The kernel is the fundamental component of an operating system for computers (OS). The OS's other aspects are dependent mostly on core to provide them with essential services.
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While loop project
Reference codeacademy lesson called loops
Need help writing this code
Create a program that
1) outputs the name priya 100 times (all on different lines)
2) output the name priya 100 times (10 on 10 lines)
3) output the sum of numbers from 1 to 1000
4) output the product of numbers from 1-8
5) list all the numbers from 100 down to 1
Complet in this order
Answers
Answer: Change this however you'd like :)
Explanation:
for n in range(100):
print("priya")
print()
for n in range(10):
for i in range(10):
print("priya", end= " ")
print()
print()
tempList = []
for n in range(1, 1001):
tempList.append(n)
print(sum(tempList))
print()
for n in range(1, 9):
for i in range(1, 9):
print(n*i)
print()
x = 100
while x != 0:
print(x)
x -= 1
Please answer quick:))))
Answers
Which keyboard shortcut can you use to insert or modify a hyperlink in a document?.
Answers
The keyboard shortcut that you you use to insert or modify a hyperlink in a document is Ctrl+K.
How are links added and changed?Anywhere on the link, perform a right-click and select Edit Hyperlink from the shortcut menu. Select the text in the Text to display box of the Edit Hyperlink dialog. Click OK after entering the text you want to use as the link.
Note that Ctrl+K Create a link here. The line or selected text is aligned to the left of the screen by pressing Ctrl+L. The paragraph will be indented using the keyboard shortcut M. Pressing the keys N and N opens a brand-new document window.
Hence, Input Ctrl+K. You may also choose Link from the shortcut menu by right-clicking the text or image and selecting it. Type or paste your URL into the Address box of the Insert Hyperlink box.
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Which of the following is an example of data a scientific research satellite would collect?
a. chemical resources in the United States
b. locations of aircraft in the atmosphere
c. atmospheric condition of the United States
d. composition of space near the earth
Answers
Answer:
d. Composition of space near the earth
d
Explanation:
hope it helps good lucjlk
TCP uses what two mechanisms to recover from errors and lost data (Choose two)? Salutations numbers Acknowledgement numbers Reply numbers L Response numbers "I Series numbers Sequence numbers
Answers
Error control includes a system for addressing issues when they are discovered. Checksum, acknowledgment, and time-out are the three simple detection and correction of errors techniques available in TCP.
Which protocol does the TCP/IP suite employ for transmission?The transfer of information from sender towards receiver is precisely defined by TCP/IP. The User Data Gram Protocol (UDP) or even the Transmission Control Protocol, for short, are the first Internet Tls Protocols that application programmes use to convey messages or streams of data (TCP).
What is the primary purpose of TCP/IP?A group of communication protocols called TCP/IP, or the Transmission Control Protocol/Internet Protocol, are used to connect network devices on the internet. Moreover, a private network uses TCP/IP as its communication mechanism.
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What is CPU known as in the computer?
Answers
Answer:
It is also known as the processor of the computer
Explanation:
The CPU is what retrieves and executes instructions, causing it to oftenly be referred to as processor.
Answer:
It is known as the brain of the computer
Explanation:
CPU stands for central process unit. It is responsible for processing the information to the computer just like how human brains process the information in their brains first then act. Thus, why CPU is known as the brain of the computer.
Hope this helps!
The ____ function returns true when the end of the file has been reached. The ____ function returns true if a translation or other logical error has occurred on a stream. The ____ function returns true when something has happened to the physical medium of a stream.
Answers
espero te ayude yo creo que no pero ahi te va
Explanation:
¿Qué función devuelve verdadero si alguno de sus argumentos es verdadero?
FUNCIÓN O. Descripción: Comprueba si alguno de los argumentos es VERDADERO y devuelve VERDADERO o FALSO. Devuelve FALSO si todos los argumentos son FALSO.
What does music mean to you? Is it a big part of your life, or is it just "there". Answer in at least two complete sentences.
Answers
Answer:
Music means a lot to almost everyone and plays a significant role in most people's lives. With all of the different genres, music encompasses a wide range of moods and emotions, and there is something for almost everyone.
Answer:
Music plays a crucial role in several people's lives. There is proof that music has helped benefit people's lives to be more positive, and some studies show that students that listened to music and meditated with music during school, had anxiety levels less than students who didn't get such an opportunity.
Explanation:
(I've read a paper somewhere for school for a health project abt stress and anxiety)
5. Write
the
steps.
open
MS-
powerpoin
to
Answers
Answer:
Explanation:
1. Go to start ·
2. Then click on 'All programs' ·
3. Then go to Microsoft office ·
4. Click on 'Power point .
Hope it helped you. Plz mark me brainliest.
Binary is best interpreted by a computer because
it is a simple system using patterns of three numbers.
the numbers in the system represent the on and off positions of the switches in a computer’s hardware.
it is broken down into bits.
it can be easily converted to the decimal system.
Answers
Answer:
Binary is best interpreted by a computer because the numbers in the system represent the on and off positions of the switches in a computer’s hardware.
Explanation:
The rest can be ruled out because:
a) binary is not a system using patterns of three numbers, but rather two numbers
c) it's true, but it is not the direct and clear answer as to why binary is best interpreted by a computer
d) that gives extra steps for the computer and thus contradicts the fact
Answer: B. the numbers in the system represent the on and off positions of the switches in a computer’s hardware.
Leslie has not properly bugeted for savings, retirement, or debt repayment
Answers
Savings, debt repayment, and retirement are the areas of Leslie's budget that are being negatively impacted by her underspending.
What happens if you don't budget correctly?A financial projection of a person, business, or government's earnings and expenses is simply referred to as a budget.It should be emphasized that the areas of Leslie's budget that her underspending is impacting include savings, debt reduction, and retirement. The most frequent effects of not budgeting are, in brief, a lack of savings, diminished financial stability, unrestrained spending, a greater risk of incurring debt, and increased financial stress. It will be challenging to live the same lifestyle in retirement as you had while working if you don't have any money.You might need to make changes like downsizing your house or apartment, giving up luxuries like cable television, an iPhone, or a gym membership, or driving a less expensive vehicle.To learn more about budget refer
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This program has a bug. When the green flag is clicked, nothing happens.
How would you fix it?
Answers
Connect the event block directly to the “repeat” block *if it is Scratch that is)
what tells the hardware what to do and how to do it?
Answers
Answer:
Software is used to to tell hardware what to do and how to do.
Explanation:
Software is a program that helps to perform various tasks from hardware.
The answer is D!!Readable code includes
o the use of formatting to make the code look better.
the use of dense lines of code without indentation.
o the use of comments that are of no use to future users of the progran
Answers
Answer:
D
Explanation:
says in title
Readable code includes the use of comments that are of no use to future users of the program. The correct option is c.
What are readable codes?Readable code is just code that makes its goal crystal plain to the reader. The likelihood is that other developers will read the code we write and want to either comprehend it or modify it. It's interesting to read the code. Variable, function, and class names in the code are meaningful.
The code may need to be tested, a bug fixed, or a new feature added. The evolution of the codebase is facilitated by readable source code, which makes it easier to read and comprehend the abstraction phases. Future developers can save time and effort by writing readable code.
Therefore, the correct option is c, the use of comments that are of no use to future users of the program.
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In which place does essential computing of computer takes place at?
option
1]microprocessor
2]ram
3]motherboard
4]none
Answers
Answer:
123456789-0\(\lim_{n \to \infty} a_n fffff\)
Explanation:
what tools can data analysts use to control who can access or edit a spreadsheet? select all that apply.
Answers
The tools that data analysts can use to control who can access or edit a spreadsheet include:
1. Password Protection: By setting a password, data analysts can restrict access to the spreadsheet and ensure that only authorized individuals can open and modify it. 2. User Permissions: Spreadsheet applications often provide options to assign different user permissions, such as read-only access or editing rights. Data analysts can assign specific permissions to different users or user groups to control their level of access and editing capabilities. 3. File Encryption: Encrypting the spreadsheet file adds an extra layer of security, ensuring that only authorized individuals with the decryption key can access and modify the data. 4. Version Control Systems: Version control systems allow data analysts to track changes made to the spreadsheet and manage different versions. This helps in controlling who can make edits and provides the ability to revert to previous versions if needed. 5. Cloud Collaboration Tools: Cloud-based spreadsheet tools often offer collaborative features that allow data analysts to share spreadsheets with specific individuals or teams. These tools provide granular control over access rights and editing permissions. By using these tools, data analysts can effectively manage and control access to their spreadsheets, safeguarding the integrity and confidentiality of the data.
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I Need help please
I will give BRAINLIST for correct answers only
Answers
Historically, the only interface to manage and execute jobs on an ibm operating system was keypunch cards. the popular online interface to access ibm operating systems, manage jobs, perform system program tasks is called: _________
Answers
The popular online interface used to access IBM operating systems, perform system program tasks, and manage jobs is called 'System i Navigator'.
System i Navigator is the primary user interface used to access and manage IBM environments. System i Navigator provides a graphical interface, with that you can manage and administer your IBM systems. System i Navigator allows to perform tasks to manage the IBM operating system through an Internet Web browser.
Historically, the only interface to manage and execute tasks on an IBM operating system was keypunch cards. However, now 'System i Navigator' provides the popular online interface to access IBM operating systems, perform system program tasks, and manage jobs.
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PLEASE HELP SOON!!!!!
What does a sort tool enable you to do in a database?
remove irrelevant data from a form
hide irrelevant data in a form
arrange records by number of typos entered in a field
arrange records by field in a form
Answers
Answer:
DExplanation:
arrange records by field in a formHow do we know if we can believe the things on the internet?
Answers
Answer:
you have to check for reliability
Explanation:
Answer:
you can't always believe the internet some stuff is false are bended
Explanation:
yea
An operation that copies a value into a variable is called a(n) ________ operation.
a. equals
b. copy
c. declaration
d. cout
e. assignment
Answers
Answer:
e
Explanation:
the first step in the communication process is: select one: a. encoding the message and selecting the transmission channel. b. transmitting the message through a channel. c. decoding the message and deciding whether the feedback is needed. d. transmitting feedback, a response, or a new message through a channel.
Answers
The correct answer is A. Encoding the message and selecting the transmission channel is the first step in the communication process.
The communication process involves a sender, a message, a receiver, and a medium or channel through which the message is transmitted. In order to initiate the communication process, the sender must first encode the message into a form that can be transmitted through the chosen channel. This may involve converting the message into a particular language, selecting specific words, or using symbols or images to convey the message.
Once the message has been encoded, the sender must select an appropriate transmission channel to deliver the message to the receiver. The choice of the channel may depend on factors such as the distance between sender and receiver, the urgency of the message, the reliability of the channel, and the cost of using the channel.
After the message has been encoded and the channel has been selected, the sender can then transmit the message through the chosen channel. This begins the process of message reception, decoding, and response by the receiver. Ultimately, the success of the communication process depends on effective encoding, channel selection, and message transmission, as well as accurate decoding and appropriate feedback.
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How can I learn programming in mobile?
Answers
Answer:
You sadly cannot use your coding knowledge to complete things on mobile, but you can learn through YouTuube and Gooogle and such, and simple type it out in Gooogle docs or something. There are sadly no mobile friendly coding websites.
Hope This Helped!
What is output? Select all that apply. c = 0 while (c < 5): c = c + 1 print(c) 6 5 2 4 3 0 1
Answers
Answer:
2 4 3 0 1
Explanation: