Alkaline lysis or alkaline extraction is a method used
in molecular biology to isolate plasmid DNA from bacteria. Please
explain the principle of how solution I, II and III work in
alkaline lysis.

Answers

Answer 1

The principle of alkaline lysis involves the use of Solution I to create an isotonic environment, Solution II to lyse the bacterial cells, and Solution III to neutralize the alkaline conditions and precipitate the plasmid DNA. This method allows for the efficient isolation of plasmid DNA from bacterial cells for further molecular biology applications.

Alkaline lysis is a method used in molecular biology to isolate plasmid DNA from bacteria. The method involves a sequence of steps using various chemical solutions, where the bacteria are first lysed and then the plasmid DNA is separated from other cellular components.

The principle of how solutions I, II, and III work in alkaline lysis is explained below:

Solution I: The initial step in alkaline lysis is to resuspend bacterial cells in a buffer solution known as a solution I, which contains Tris-HCl, EDTA, and glucose. Tris-HCl acts as a buffering agent to maintain the pH of the solution, while EDTA chelates metal ions that could interfere with DNA isolation and glucose serves as a source of carbon for the bacteria.

Solution II: After centrifugation of the bacterial cell suspension, solution II is added to the pelleted cells, which contain sodium hydroxide (NaOH) and sodium dodecyl sulfate (SDS). NaOH denatures the bacterial proteins and breaks the hydrogen bonds between the base pairs of the DNA, resulting in the lysis of bacterial cells and the release of their contents. SDS acts as a detergent to solubilize the bacterial cell membranes, and the resulting solution is highly alkaline, with a pH of 12-13.

Solution III: The final step in the alkaline lysis process is to add a neutralizing buffer, known as solution III, which contains potassium acetate. Potassium ions neutralize the high pH of the solution, allowing the DNA to re-anneal and form a complex with the SDS, which precipitates out of the solution. Other cellular components, such as RNA, proteins, and cell debris, remain in solution. The precipitated plasmid DNA can then be collected by centrifugation, washed with ethanol, and resuspended in a buffer of choice.

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Related Questions

Match the following DNA strand to its complementary strand of rna

Match the following DNA strand to its complementary strand of rna

Answers

Answer:

AUG - GGC - UUA - AGC

Explanation:

Thymine turns into Uracil in an RNA transcript. Meaning Adenine (A) and Uracil (U, T from the DNA strand) go together, and Guanine (G) and Cytosine (C) go together to create the chromatid.

what shape and color were the acid-fast cells

Answers

The acid-fast cells are rod-shaped and are red-orange in color. This is due to the presence of a waxy substance called mycolic acid that coats the outside of the cell and makes them resistant to acid decolorization. The mycolic acid is also responsible for giving the cell its distinctive red-orange color.

Acid-fast cells are bacteria that have a cell wall made up of lipids and proteins that make them resistant to certain acids and detergents. They are usually rod-shaped, but can also be cocci or oval shaped. They are usually red or pink in color due to the presence of carotenoid pigments in their cell walls.

Step 1: Acid-fast cells are bacteria that have a cell wall made up of lipids and proteins.

Step 2: These bacteria are usually rod-shaped, but can also be cocci or oval shaped.

Step 3: The cell wall of the acid-fast cells contains carotenoid pigments, which give the cells a red or pink color.

Step 4: These bacteria are resistant to certain acids and detergents due to the presence of lipids and proteins in their cell wall.

Step 5: In conclusion, acid-fast cells are usually rod-shaped bacteria, with a red or pink color due to the carotenoid pigments in their cell walls. They are resistant to certain acids and detergents due to the lipids and proteins in their cell walls.

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Please help i am giving away brainiliest
Which of the following is necessary in order for natural selection to act on a
population?

A. selective pressures that favor certain individuals
B. asexual reproduction through mitosis
C. equal chance of survival for all individuals
D. decrease in diversity through genetic drift

No dam links

Answers

Answer:

A

Explanation:

Natural selection occurs when an environment makes organisms with certain traits more likely to survive and reproduce.

Asexual reproduction results in no genetic variation (meaning differences in traits, also called divserity). Genetic variation must be present for certain organisms to be better suited to their environment, because in its absence all organisms share the same traits. This rules out B, C, and D. I hope this helps :)

Selective pressures that favor certain individuals are necessary in order for natural selection to act on a population. Therefore, option (A) is correct.

What is natural selection?

The process by which organisms are able to pass on the favorable qualities they possess to their offspring is known as natural selection. Organisms that are better able to adapt to their surroundings have a greater chance of surviving and reproducing in that environment. As a consequence of this, advantageous characteristics become more prevalent over time, whereas undesirable traits become less prevalent, resulting in a change in the genetic make-up of a population.

Natural selection can be influenced by a variety of environmental factors, including predation, competition for resources, and shifts in climate. It is a fundamental mechanism of evolution and contributes to our understanding of the diversity of life on Earth as well as the various ways in which different species have adapted to their surroundings.

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will mark brainly please help

will mark brainly please help

Answers

Answer:

The answer is

Explanation:

Coronal mass ejections match with energy released during nuclear fusion.

Solar wind match with includes prominences and solar flares.

Gamma ray photon match with a stream of plasma.

Hope this helps....

Have a nice day!!!!

Answer:

Coronal mass ejection: a stream of plasmaGamma ray photon: energy released during nuclear fusionSolar wind: includes prominences and solar flares

can a person have more than 46 chromosomes? what would be the results if this hapened

Answers

Answer:

Yes, they can. Birth defects can impact a chromosone count. People with down syndrom have more than 46 chromosones and it occurs when someone is born with a full or partial extra copy of chromosome 21.

Explanation:

Cerebellar lobes are comprised of multiple enfoldings called _______________

Answers

Cerebellar lobes are comprised of multiple enfoldings called folia.

All vertebrates have a cerebellum, which is a prominent component of the hindbrain. In some animals, such as the mormyrid fishes, it may be as large as or even larger than the cerebrum, despite the fact that it is typically smaller. The cerebellum is a key component of human motor control.

Although its movement-related functions are the most well-established, it may also be involved in some cognitive processes like attention and language as well as emotional control processes like regulating fear and pleasure responses. The human cerebellum does not start movement, but it does help with coordination, precision, and correct timing by receiving information from other areas of the brain and from sensory systems in the spinal cord and integrating it to control motor activity.

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photosynthesis uses raw materials for converting radiant energy into a more usable form. what is the usable form of energy produced by this process?
Carbon dioxide, a form of thermal energy
Oxygen, a form electrical energy
Cellulose, a form of electrical energy.
Glucose, a form of chemical energy.

Answers

Photosynthesis uses raw materials for converting radiant energy into a more usable form. The usable form of energy produced by this process is Glucose, a form of chemical energy.

What is Photosynthesis?

Photosynthesis is the process of making food by converting carbon dioxide and water into carbohydrates, using radiant energy from sunlight. During photosynthesis, radiant energy is converted into a more usable form of energy by using raw materials such as water and carbon dioxide. Photosynthesis is an important process because it provides oxygen and carbohydrates.

During photosynthesis, the radiant energy is trapped in the chloroplasts and stored as chemical energy in the form of glucose or other carbohydrates, which can be used later by plants for various metabolic processes.

What is the usable form of energy produced by photosynthesis?

The usable form of energy produced by photosynthesis is Glucose. It is a simple sugar that provides energy to the cells. Glucose is produced by photosynthesis by combining carbon dioxide and water. Glucose is a carbohydrate and is one of the most important sources of energy for living organisms. The glucose produced by photosynthesis is used by plants to provide energy for their own metabolic processes, while the excess glucose is stored as starch, which can be used later when needed.

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Exons are parts of genes that code for specific proteins.a. Trueb. False

Answers

Exons are parts of genes that code for specific proteins. (TRUE)

About Exon

Exons are the part of a gene that codes for an amino acid. In plant and animal cells, most gene sequences are broken down by one or more DNA sequences called introns.

The parts of the gene sequence that are expressed in proteins are called exons, because they are expressed, while the parts of the gene sequence that are not expressed in proteins are called introns, because they are located between – or interfering with – exons.

Exons are the part of the RNA that codes for the protein. Now, RNA, when it was first transcribed, was a very, very long part of the RNA molecule. And really, the important parts of RNA are called exons.

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Which of the following is a cellular structure responsible for protein production

Answers

You did not put any choices, but ribosomes and the Rough endoplasmic reticulum (RER) are both responsible for protein production.

I’m looking for the answer q 1 and 2 please

Im looking for the answer q 1 and 2 please

Answers

Answer:

wfhhtsgbjjjhtddhhtdvnkkkkrwCbjyxcsrthxwry6nbxefvtynvggy

Here is what use the help
Im looking for the answer q 1 and 2 please

Which describes nuclear fission?(1 point)
Responses

starts when a neutron slams into an atom, causing two atoms to form one, heavier atom
starts when a neutron slams into an atom, causing two atoms to form one, heavier atom

starts when two atoms slam together to form one, heavier atom
starts when two atoms slam together to form one, heavier atom

starts when two atoms slam together, causing the larger atom to split into two smaller atoms
starts when two atoms slam together, causing the larger atom to split into two smaller atoms

starts when a neutron slams into an atom, causing the larger atom to split into two smaller atoms

Answers

The option that describes nuclear fission is option C: starts when a neutron slams into an atom, causing the larger atom to split into two smaller atoms

What is nuclear fission?

Nuclear fission is a process in which the nucleus of an atom is split into two or more smaller nuclei, along with the release of a large amount of energy. This process starts when a neutron (a subatomic particle) collides with the nucleus of a heavy atom, such as uranium or plutonium.

Therefore, When this happens, the nucleus becomes unstable and splits into two or more smaller nuclei, releasing a large amount of energy in the form of heat, light, and radiation. This energy is harnessed in nuclear power plants to generate electricity. Additionally, the process also releases more neutrons, which can then go on to cause further fission reactions, creating a chain reaction.

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Anyone know the answer?

Anyone know the answer?

Answers

Answer:

Behavior

Explanation:

It would be an innate behavior such as a reflex action: an involuntary and rapid response to a stimulus, or cue.

No I don’t know at all

PLEASE NEED HELP!!!!

PLEASE NEED HELP!!!!

Answers

Outer membrane: A
Grana: B
Thylakoid: C

Phosphorus forms part of the important life-sustaining molecules such DNA and RNA
True or false

Answers

Answer:

False

Explanation isn’t phosphorus a chemical?

Answer:

true

Explanation:

Phosphorus is the most abundant gas in the atmosphere. Organic phosphate is taken up by producers during photosynthesis and released by cellular respiration. Phosphorus forms part of the important life-sustaining molecules such as DNA and RNA. Plants absorb phosphorus from the atmosphere or water.

which subset of the following is directly involved in driving protein import into the mitochondrial matiex space?

Answers

The subset directly involved in driving protein import into the mitochondrial matrix space is the translocase of the outer mitochondrial membrane (TOM) complex.

The TOM complex consists of various subunits, including Tom20, Tom22, Tom40, and Tom70, which are responsible for recognizing and binding to precursor proteins with specific targeting signals. The Tom40 subunit forms a channel through which the precursor proteins can pass into the intermembrane space, while the Tom22 subunit interacts with the TIM23 complex in the inner membrane to facilitate transport into the matrix. Additionally, the Tom70 subunit plays a role in the import of membrane proteins. The TOM complex is crucial for the efficient and specific targeting of precursor proteins to the mitochondria and is thus an essential component of the protein import machinery.

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into the correct order from smallest to largest ( organ, organ system, organelle,tissue,cell, organism​

Answers

Organelle, cell, tissue, organ, organ system, organism.

why is the sky black​

Answers

Answer: The sky appears black at night due to the absence of sufficient light reaching our eyes from distant stars and galaxies.

Explanation:

The sky appears black during the night when there is no significant source of illumination. This phenomenon is known as the "night sky paradox." The reason behind the blackness of the night sky is primarily attributed to the vastness of the universe and the limitations of human vision.

During the day, the sky appears blue because of a process called Rayleigh scattering. The Earth's atmosphere scatters shorter blue wavelengths of light more than longer wavelengths, creating the blue color we observe.

At night, when the sun is no longer visible, our line of sight extends deeper into space. We can observe countless stars in various directions, implying that there should be light coming from all those stars. However, the night sky still appears dark overall.

The reason for this darkness is that the universe is not infinitely old. It has a finite age. Consequently, the light from the most distant stars and galaxies has not had enough time to reach us yet. As a result, the vast majority of the universe is beyond our observational reach, and we perceive the night sky as predominantly black.

In addition, there are other factors that contribute to the blackness of the night sky, such as interstellar dust, which absorbs and scatters light, and the absorption of light by gases and particles in our atmosphere.

Therefore, the sky appears black at night because the light from distant stars and galaxies has not reached us, and the absence of significant illumination sources contributes to the overall darkness.

In the United States, alcoholic beverages that are sold in stores display a government warning stating that pregnant women should not drink alcoholic beverages. This is because it prevents cleavage division from occurring, so a zygote cannot become a blastocyst. if a woman drinks alcohol while she is pregnant, she may not be able to ovulate again after childbirth. studies have proven that alcohol consumption causes long-lasting disorders in the fetus. the science of chemistry has shown that alcohol is a reactive substance that may interfere with the mother’s food digestion.

Answers

Answer:

The correct answer is - studies have proven that alcohol consumption causes long-lasting disorders in the fetus.

Explanation:

Alcohol can cross the barrier that the placenta creates between the mother and fetus so the alcohol can negatively impact the fetus or newborn by various means.

It can harm the fetus as it may result in damage to the cells of the fetus. Brain cells and spinal cord cells are prone to such kind of alcohol toxicity or syndrome. Alcohol spectrum disorder that affects the development of the baby which could be long-lasting.

Answer:

studies have proven that alcohol consumption causes long-lasting disorders in the fetus.

Explanation:

Which type of evidence acts as a record of changes in species over time and indicates major changes in Earth's surface and climate? A. Fossil record B. Movement of continents C. Ripple marks and mud cracks D. Composition of ice core samples

Answers

Answer:

A. Fossil record

Explanation :

A fossil can be defined as any preserved element (e.g., bones, exoskeletons, shells, animal imprints) which can be used to demonstrate the existence of organisms from past geological periods. The totality of fossils accumulated in a particular area is referred to as the fossil record. The fossil record is very useful in geology and paleontology because the information provided by the fossils helps to place geological events and species in their corresponding geologic eras and periods.

Answer:

Fossil Record

Explanation:

What are the definitions of compression and tension

Answers

Answer:

Compression means a decrease in the volume of any object

Tension is the degree to which anything is stretched or strained

Explanation:

Hope this helps:).....if not then sorry for wasting your time and may God bless you:)

How does microscopes help us learn about the structure and function of unicellular and multicellular organisms?

Answers

Microscopes play a crucial role in helping us learn about the structure and function of both unicellular and multicellular organisms. These specialized instruments magnify small objects, allowing scientists to observe.    

Analyze the detailed characteristics of living organisms that are otherwise invisible to the eye. For unicellular organisms, such as bacteria and protozoa, microscopes enable researchers to study their shape, size, and internal structures, which are vital for understanding how these organisms function, reproduce, and interact with their environment. The knowledge gained from these observations has significant implications for fields like medicine, environmental science, and biotechnology. In the case of multicellular organisms, microscopes allow for the examination of various cell types, tissues, and organs. This helps scientists understand how cells work together to perform specific functions and maintain the overall health of the organism.

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differentiate between active natural immunity and passive natural immunity?​

Answers

Explanation:

A prominent difference between active and passive immunity is that active immunity is developed due to the production of antibodies in one's own body, while passive immunity is developed by antibodies that are produced outside and then introduced into the body.

Does every muscle have the ability to have a concentric and eccentric muscle contraction?

Answers

Answer:

Eccentric muscle contraction is muscle lengthening as the muscle produces tension and the insertion moves away from the origin.

pls brinley meeeeeeeeeee

which represents the amount of potential food available at each tropic level

Answers

Answer:

the biomass pyramid

Explanation:

A biomass pyramid represents the amount of potential food available for each tropical level in the ecosystem.

How important is DNA to Law enforcement? Should arrested (not convicted Individuals) be forced to provide DNA samples to the police?

Answers

DNA is incredibly important to law enforcement for several reasons. It serves as a valuable forensic tool that can be used to identify individuals, establish connections between suspects and crime scenes, and provide crucial evidence in criminal investigations.

DNA analysis has revolutionized the field of forensic science and has helped solve numerous cases, including cold cases and wrongful convictions.

However, the question of whether arrested individuals (not convicted) should be forced to provide DNA samples to the police is a complex ethical and legal issue. Different jurisdictions have different laws and regulations regarding the collection and retention of DNA samples from arrested individuals.

Supporters of DNA collection from arrestees argue that it can aid in solving crimes and preventing future offenses by identifying potential repeat offenders or linking them to unsolved cases. They contend that DNA collection at the time of arrest is analogous to other routine identification procedures like fingerprinting and photographing, which are commonly accepted and practiced.

On the other hand, opponents argue that requiring DNA samples from arrested individuals infringes upon their privacy rights and presumption of innocence. They express concerns about potential abuse, such as the misuse of DNA samples or the expansion of databases beyond the scope of serious crimes. Additionally, there are concerns about the disproportionate impact on marginalized communities and the potential for racial or ethnic profiling.

The approach to DNA collection from arrested individuals varies across jurisdictions, reflecting differing legal and constitutional considerations. Some countries or states allow DNA collection upon arrest for certain serious offenses, while others restrict it to convicted individuals or limit it to specific circumstances.

Ultimately, the decision regarding DNA collection from arrested individuals involves balancing the need for effective law enforcement with protecting individual rights and privacy. It requires careful consideration of the legal framework, scientific advancements, societal values, and potential risks associated with the use and retention of DNA samples.

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Of the following, which is NOT considered to increase the risk of developing cancer for humans?Select one:a.ultraviolet lightb.toxic chemicalsc.regular exercised.smoking

Answers

Answer is C.

Explanation: Exercise is good for the body. It helps regu

GIVING BRAINLIEST, THANKS AND FIVE STARS THANK YOU HAVE A GREAT DAY!!!!!!!!!!!!!

The eggs of a cone bearing plant:

receive sperm through the development of tubes

are held on the sticky fluid of the female cone

receive pollen from the wind

Answers

Answer:

Cone-bearing plants have two types of cones: female cones and male cones. Reproduction begins when one of the pollen grains from inside a pollen sac on the male cone floats through the air to the scale of a female cone. A pollen tube then grows from the pollen grain toward the female ovule.

All living things are able to
environment.
to things within their own

Answers

Answer: Bio

Explanation: Bio means living

.Diagrams depicting family relationships and phenotypes for a genetic disorder areA. linkage maps.B. pedigree charts C. genotype maps.D. Punnett squares.E. bell-shaped curves.

Answers

Diagrams depicting family relationships and phenotypes for a genetic disorder are pedigree charts. Here option B is the correct answer.

A pedigree chart is a diagram that depicts family relationships and the inheritance of a genetic disorder over several generations. It is used to trace the inheritance of a particular trait or disease within a family and can help to identify patterns of inheritance.

The chart typically includes symbols for males and females, as well as symbols to indicate the presence or absence of a particular trait or disorder. It also can indicate the genotype of the individual, whether they are carriers or affected by the disorder.

A linkage map is a diagram that shows the relative positions of genes on a chromosome, and the distance between them. A genotype map is a diagram that shows the genetic makeup of an organism.

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Today, which of the following regions relies most on the importation of food?

Answers

asia and africa as they both use the most on the importation of food to support their communities
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